Figure 2.

Effect of NOS inhibitor and NO donor on IL-1-stimulated bone resorption in WT BM cocultures. (a) Resorption surface area. (b) Number of osteoclasts per dentine slice. Recombinant human IL-1β (10 units/ml), the NOS inhibitor N-monomethyl arginine (NMMA; 1 mM), or vehicle was added at day 7. The NO donor SNAP (5 μM) was added on days 7, 8, and 9, and the cultures were terminated on day 10. *, P < 0.05 from vehicle; **, P < 0.01; ***, P < 0.001; n = 6. The results shown are of a representative experiment of three performed.