Figure 4.

Effects of SNP on the expression of genes induced by wounding, systemin, and JA. Young excised tomato plants were supplied with phosphate buffer alone (control) or 1.0 mm SNP for 1 h. Plants, except controls, were wounded, transferred to water, and assayed by RNA gel blotting after 2 h for allene oxide synthase, lipoxygenase, prosystemin, and polygalacturonase catalytic subunit (and after 8 h for proteinase Inh), proteinase Inh II, cathepsin D inhibitor, and metallocarboxypeptidase inhibitor. Equal amounts of RNA were loaded as confirmed by probing with an ubiquitin cDNA.