Figure 7.

Identification and chromosomal localization of AFLP markers linked to the Def-1 gene. AFLP analysis for Def-1-linked markers was performed on bulked segregants from a BC₁ segregating population. Four genomic template DNAs were used for each primer combination: P⁻, W⁻ parent (L. esculentum, def-1/def-1); P⁺, W⁺ parent (L. pennellii, Def-1/Def-1); B⁻ and B⁺, bulks composed of 10 W⁻ and 10 W⁺ progeny, respectively, from a BC₁ mapping population (see text for details). A, Arrows indicate the position of the EM-1 (top) and EM-2 (bottom) AFLP markers identified using two different primer combinations. Only the portion of the autoradiographed AFLP gel is shown. B, Conversion of EM-1 and -2 to RFLP markers and mapping of the markers to IL3-5. Cloned AFLP markers were labeled with ³²P and hybridized to genomic DNA digested with HindIII (EM-1, top) or HaeIII (EM-2, bottom). Genomic DNA was obtained from plants with the following genotypes: lane 1, F₁ hybrid (Def-1/def-1) between L. esculentum (def-1/def-1) and L. pennellii (LA716); lane 2, L. esculentum (def-1/def-1) parent; lane 3, L. pennellii (Def-1/Def-1) parent; and lane 4, IL (LA3490) harboring the IL3-5 segment of L. pennellii DNA.