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. 2002 Oct;130(2):591–604. doi: 10.1104/pp.005637

Figure 8.

Figure 8

In situ localization of Ivr2 soluble invertase mRNAs in tissues of young maize kernels (NK-508) at +6 d post-pollination. Labeled Ivr2 mRNAs are evident in several areas of the maternal tissues that make up the maize kernel at this stage of development, with strongest signal in cells surrounding the tiny, newly fertilized embryo + endosperm (minute and off the field of view; see drawing to lower right) and in cells near vascular bundles of the pedicel. Signal proximal to the embryo + endosperm is localized in basal regions of the nucellus (maternal tissue filling the central kernel during early development), plus cells of the lower pericarp (ovary wall). Individual kernels were sampled as soon as pollinated ovaries could be visually distinguished from non-pollinated neighbors. Fixed and sectioned samples were probed with sense (control, top right) and antisense (in situ localization) RNA probes synthesized with digoxygenin-labeled UTP from a 576-bp Ivr2 fragment as described in the text.