Figure 5.

The cysts/CHIBs are responsive to glucose in vitro secretion studies. Forty cysts/CHIBs were incubated in six replicates from each of two groups for three pancreases. After an initial 4-h preincubation, each sample was incubated for 24 h in RPMI with 5 mM glucose for basal secretion determination. The media were replaced with either fresh media with 5 mM (hatched bars) or 20 mM (solid bars) glucose for an additional 24 h. Insulin secretion over the 24-h period was expressed as percent of the same tube at basal. Parallel experiments with middle layer tissue from two flasks of pancreas H99–19 and of H99–25 and one flask from H99–24 are shown. Insulin content was not determined.