Table 1.
Insulin and DNA content of 75-cm2 flask containing cultured human ductal cells
| Original aliquot, 50 ml | Initial adherent | 2 wk | 3 wk | 4 wk | |
|---|---|---|---|---|---|
| H99-13 (top + middle, 58% islet) | |||||
| Insulin, ng | 3,200 | 78.4 | — | 888 | — |
| DNA, μg | 160 | 3.8 | — | 22.1 | — |
| I/DNA, ng/μg | 20 | 20.5 | — | 40.2 | — |
| H99-19 (middle, 5% islet) | |||||
| Insulin, ng | nd | 70.8 | 123 | 344 | 863 |
| DNA, μg | nd | 30.7 | 39.8 | 29.8 | 41 |
| I/DNA, ng/μg | 2.3 | 3.1 | 11.5 | 21.1 | |
| H99-20 (middle, <5% islet) | |||||
| Insulin, ng | 1,600 | 174 | — | 1,788 | 2,564 |
| DNA, μg | 250 | 60 | — | 42.9 | 46.8 |
| I/DNA, ng/μg | 6.4 | 2.9 | — | 41.2 | 54.8 |
At 2–4 days after islet isolation the majority of the tissue aliquot originally placed into the culture flask was removed, leaving only the tissue adhering to the nontreated surface. Much of the original tissue died as would be expected for acinar tissue. The cell clumps spread to form monolayers; at 2–3 weeks, these monolayers were coated with a thin Matrigel layer. nd, Not determined.