Table 2.

Enrichment of insulin content in cysts/CHIBs

Tissue origin		Insulin/DNA (ng/μg), individual samples	Insulin/DNA (ng/μg), mean ± SEM
H99-08	Top	852, 333, 249, 327	440  ± 139
H99-10	Top	48, 66	57
H99-12	Top	149, 61, 149	120  ± 29
H99-13	Top + middle	178, 218, 68	154  ± 45
H99-19	Middle	46, 110	78
H99-20	Middle	110, 152, 94, 27, 74	91  ± 21

Starting at 10 days after Matrigel, some cysts/CHIBs could be collected after becoming dislodged during media changes. Other samples were harvested at the end of an experiment by mechanical shearing. The time of culture for the cysts/CHIB samples was between 5 and 6 weeks (range 27 to 65 days) after isolation, 2–3 weeks after Matrigel (range 10 to 41 days).