Figure 3.

In situ analysis of TM29 expression in different tissues in wild-type (WT) tomato. A, Sympodial bud in the axil of a leaf showing TM29 expression at the tip. B, Bifurcating structure with a floral (fm) and an inflorescence (im) meristem. TM29 is expressed uniformly in the floral meristem and strongly at the tip of the inflorescence meristem. Transcripts are also seen in the vascular bundles (vb). C, Tissue section as in B probed with sense RNA as negative control. D, Section showing floral meristems with emerging sepal primordia at different stages. E, Tissue section as in D probed with sense RNA as negative control. F, A floral meristem with elongated sepals. TM29 expression in the sepals is markedly reduced at this stage. G, Flower bud with all four floral organ primordia. TM29 expression is reduced in the petals and is localized to the tip of the stamens where the anthers (an) are formed. Expression continues to be seen in the center of the ovary (ov). H, Flower bud at about 4 d pre-anthesis. TM29 expression is localized to the pericarp (per) region of the ovary and in the tapetum (tap) regions of the stamens. an, Anther region; fm, floral meristem; im, inflorescence meristem; lf, leaf; ov, ovary primordium; per, pericarp region; sb, sympodial bud; se, sepal primordium; st, stamen; tap, tapetal region; vb, vascular bundle. Scale bar = 1.5 mm.