Figure 7.

The expressions of sense and antisense TM29 transcripts in transgenic lines. A, Total RNA extracted from six sense transgenic plants and the WT plant was probed with antisense probe. Lane 1 contained RNA from the line S/05 showing altered phenotype. Lanes 2 through 6 contained RNA samples from five plants showing no altered phenotypes. B, Total RNA extracted from six antisense transgenic plants and the WT plant was hybridized with antisense and sense probes separately to detect endogenous sense and transgene-expressed antisense transcripts, respectively. The loading levels are shown by hybridization with rRNA gene probe. For symbols of the phenotype, refer to Table I.