Figure 4.

Deletion analysis of the CAT3::LUC promoter reveals an EE that is necessary for evening-specific circadian transcription. A, Summary of the CAT3::LUC promoter resection indicating the proportion of independent transgenic lines expressing evening-specific circadian LUC activity in LL. B, Plants were grown as described in the Figure 1 legend and released into LL. The LD regime is indicated by the bars beneath the traces, with subjective day indicated by white bars and subjective night indicated by gray bars. Traces present average values (± se, n = 12), normalized as described in the legend to Figure 2, from −1,130/+1 CAT3::LUC (blue triangles), −199/+1 CAT3::LUC (red squares), and −174/+1 CAT3::LUC (black circles) seedlings. C, Phase plots of 12 seedlings from single transgenic lines carrying either the −1,130/+1 CAT3::LUC (blue triangles) or the −199/+1 CAT3::LUC (red squares) constructs. D, Nucleotide sequence of the 25-bp CAT3 promoter region between −199 and −174, which is required for rhythmicity and contains the EE, AAAATATCT (highlighted), and the lhc motif, CAN_2–4ATC (underlined; Piechulla et al., 1998).