Figure 6.
Gain of function experiments show that a 118-bp region from the CAT3 promoter is sufficient to confer evening-specific circadian LUC activity. Plants were grown as described in the Figure 1 legend and released into LL. A, Traces present average values (± se, n = 12), normalized as described in the legend to Figure 2, from (−221/−118)2 CAT3::LUC (red circles), −281/+1 CAT3::LUC (black squares), and (−203/−163)1 CAT3::LUC (blue triangles) seedlings. The LD regime is indicated by the bars beneath the traces, with subjective day indicated by white bars and subjective night indicated by gray bars. B, Cartoon comparing CAT3 promoter fragments used in gain-of-function experiments. C, Phase plots of 12 seedlings from one transgenic line for each of the two rhythmic constructs shown in A.