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. 2002 Oct;130(2):666–674. doi: 10.1104/pp.005538

Figure 2.

Figure 2

GhTUB1 gene structure and construction of the chimeric genes between the GhTUB1 5′-flanking region and the GUS gene. The physical structure of the GhTUB1 gene was characterized in detail. GhTUB1 encodes a 445-amino acid β-tubulin polypeptide. The translated portions of exons are denoted by black boxes; introns, the 5′-flanking region (including putative promoter and 5′-UTR), and the 3′ terminus are denoted by lines. The lengths of the introns in base pairs are indicated. The number at the boundaries of each exon indicates the codon at which the intron is located. Intron 1 splits codon 132, and intron 2 splits codon 222. The translation initiation and translation termination codons are shown. The length of the 5′-flanking region and cloning sites used for fusion constructs are shown. A, GhTUB1 gene structure; B, GhTUB1::GUS fusion; aa, amino acids.