Table I.
Effects of the NO and/or H2O2 generation on lipid peroxidation
| Time | Lipid Peroxidation
|
|||
|---|---|---|---|---|
| Control | SNP | GOG | SNP + GOG | |
| h | μmol MDA g−1 fresh wt | |||
| 2 | 11.2 ± 0.8 | 13.1 ± 1.1 | 11.8 ± 0.9 | 12.3 ± 0.8 |
| 4 | 12.1 ± 0.9 | 10.8 ± 0.8 | 12.4 ± 1.1 | 18.3 ± 0.9 |
| 5 | 12.7 ± 1.0 | 11.2 ± 0.9 | 11.7 ± 0.8 | 28.2 ± 1.3 |
| 7 | 10.7 ± 0.9 | 10.2 ± 0.7 | 16.7 ± 1.2 | 26.2 ± 1.5 |
Changes over time in lipid peroxidation of tobacco cv BY-2 cells with altered levels of NO and/or H2O2 were measured as the malondialdehyde (MDA) content. The results are the means of five experiments ± se. NO and H2O2 were generated by adding SNP (0.5 mm) and/or GOG (0.5 mm Glc plus 0.5 unit mL−1 Glc oxidase) in the culture medium.