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. 2002 Oct;130(2):709–719. doi: 10.1104/pp.009993

Figure 2.

Figure 2

5′-Deletion analysis of the ProDH promoter for the l-Pro- and hypoosmolarity-responsive induction of the LUC reporter gene in transgenic tobacco and Arabidopsis plants. A, Schematics of the 5′-terminal deletions of the ProDH promoter fused to the LUC reporter gene. Arrows indicate 9-bp direct repeat sequences. B, LUC activity in transgenic tobacco plants containing 5′-terminal deletions of the ProDH promoter fused to the LUC gene. T2 seedlings of tobacco were incubated in distilled water (DW) or in 0.09 m l-Pro for 24 h. LUC activity was measured in three plants of seven independent transformant lines for each construct. Multiplication factors of induction of LUC activity (ratio of after treatment to before treatment) by DW and l-Pro treatments are shown at the right. Bars indicate se. C, LUC activity in transgenic Arabidopsis plants containing 5′-terminal deletions of the ProDH promoter fused to LUC. T2 seedlings of Arabidopsis were incubated in DW or 0.09 m l-Pro for 24 h. LUC activity was measured in seven independent transformant lines for each construct. We measured three plants for each line and showed average values.