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. 2002 Oct;130(2):709–719. doi: 10.1104/pp.009993

Figure 4.

Figure 4

Base substitution analysis of the 70-bp region from positions −730 to −661 of the ProDH promoter for the l-Pro- and hypoosmolarity-responsive induction of LUC in transgenic tobacco plants. A, Upper strand sequences of the 70-bp fragment of the ProDH promoter and its mutated sequences (M1–M8). Each fragment containing each mutation was ligated to the −42 ProDH minimal TATA promoter-LUC construct. Dashes indicate the sequence of the 3D6 construct. B, Effect of base substitutions in the direct repeat sequence for l-Pro- and hypoosmolarity-responsive expression of ProDH. T1 leaves of tobacco were incubated in DW or 0.09 m l-Pro for 24 h. LUC activity was measured in 12 to 26 leaves of independent transformant lines for each construct and is shown as average values. Bars indicate se.