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. 2002 Oct;130(2):709–719. doi: 10.1104/pp.009993

Figure 5.

Figure 5

GUS activity and histochemical analysis in transgenic Arabidopsis plants containing the 1.4-kb ProDH promoter or the 3D5 fragment fused to the GUS reporter gene after treatment with DW or l-Pro. A, GUS activity in transgenic Arabidopsis containing the 1.4-kb promoter-GUS fusion gene before and after treatment with DW or 0.09 m l-Pro for 24 h. GUS activity was measured in two independent transformant lines for each construct. We measured five plants for each line and showed average values. B, GUS activity in transgenic Arabidopsis containing the 3D5 fragment fused to the GUS reporter gene instead of the LUC reporter gene before and after treatment with DW or 0.09 m l-Pro for 24 h. GUS activity was measured in three independent transformant lines for each construct. We measured five plants for each line and showed average values. C through E, Control plants containing the 1.4-kb promoter-GUS fusion gene grown for 2 weeks and stained for 4 h. F through H, 3D5 constructs containing the GUS reporter gene instead of the LUC reporter gene grown for 14 d and stained overnight. C and F, Before treatment; D and G, after treatment with DW for 24 h; E and H, after treatment with 0.09 m l-Pro for 24 h.