Figure 2.

The loss of 48 bp of the 5′-UTR of TCH4 affects the transient accumulation of mRNA after touch or darkness. A and B, Total RNA was isolated from plants after no stimulus (0) or after touch (A) or darkness (B). Plants were harvested at the indicated times. Four micrograms of total RNA was size fractionated on gels, blotted onto nylon membranes, and hybridized sequentially with the probes shown at right. The prime indicates minutes; “h” stands for hour(s). TUB4 (Tubulin) is shown as a loading and transfer control. The transgene assayed is represented by the rectangle at the bottom (not drawn to scale); see Fig. 1 legend for details.