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. 2002 Oct;130(2):770–783. doi: 10.1104/pp.008680

Figure 3.

Figure 3

TCH4 sequences between −258 and −45 are sufficient to confer up-regulation of expression in response to heat, touch, and darkness. A, Plants harboring transgenes with TCH4 sequences between −258 and −45 (I), −958 and −45 (II) and the −90 CaMV 35S minimal promoter fused to GUS, and −958 and −1 (III) fused to GUS were unstimulated (0) or placed at 35°C. B and C, Plants harboring transgenes composed of TCH4 sequences between −258 and −45 and the 90 bp of the CaMV 35S promoter (“I”) or TCH4 sequences between −258 and −45, the 46 bp of the CaMV 35S promoter, and the TEV leader (“IV”) were left unstimulated (0) or stimulated with touch (B) or darkness (C). A through C, Plants were harvested at the indicated times. Eight micrograms of total RNA was size fractionated on gels, blotted to nylon filters, and hybridized sequentially to probes listed on the right. The lower band in the GUS panel in B and C is frequently observed and is nonspecific. The prime indicates minutes; h indicates hour(s). TUB4 is shown as a loading and transfer control. The transgenes assayed are represented by rectangles at the bottom of the figure (not drawn to scale); see Fig. 1 legend for details.