Figure 1.

Northern-blot analysis of PAL, 4CL, and COMT transcript levels in various tissues of aspen. Ten micrograms of total RNA from each tissue was resolved on a 1% (w/v) denaturing agarose gel that was photographed before being blotted and cross-linked onto a nylon membrane. Duplicate blots were hybridized with ³²P-labeled full-length PtPAL1 and PtPAL2 cDNAs, stripped between hybridizations, and consecutively probed with ³²P-labeled full-length Pt4CL1, Pt4CL2, and PtCOMT cDNAs at high stringency.