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. 2002 Oct;130(2):796–807. doi: 10.1104/pp.006262

Figure 4.

Figure 4

In situ localization of PtPAL1 and PtPAL2 mRNAs and histochemical detection of CTs in aspen root tips. Longitudinal tangential sections (A and B) and transverse sections (C–F) 10 μm in thickness were hybridized with DIG-labeled antisense PAL1 (A, C, and E) or PAL2 (B, D, and F) RNA probes. Transverse sections (75-μm thickness) were stained with DMACA for the detection of CTs (G and H). Arrows in A indicate locations of transverse sections represented in C, D, and G. Transverse sections E, F, and H correspond to a distance approximately 2 mm from root tips. Scale bar = 200 μm (A and B) or 100 μm (C–H). co, Cortex; e, epidermis; ph, phloem; rc, root cap; vc, vascular cylinder; xy, xylem.