Table IV.
Tissue-specific transcript abundance comparison between the plastid envelope, thylakoid, and stroma-localized proteins
| Envelope | Thylakoid | Stromal | |
|---|---|---|---|
| No. of predicted proteins analyzeda | 541 | 39 | 1,802 |
| No. of ESTsb | 2,349 | 1,961 | 17,970 |
| Genes with specific EST tissue distributionc | |||
| P < 0.05 | 8% (42) | 69% (27) | 19% (342) |
| P < 0.01 | 2% (13) | 51% (20) | 4% (71) |
Predicted envelope-localized proteins were from AtPEM candidates. Thylakoid proteins were selected from known thylakoid proteins and their homologs mostly involved in photosynthesis. Stromal localized proteins were randomly chosen from the predicted plastidial proteins (by TargetP) subtracted by those with membrane-spanning domains (predicted by TMHMM) and known thylakoid proteins.
No. of ESTs corresponding to the analyzed genes. The ESTs for thylakoid and stromal proteins were from TIGR Gene Index Tentative Consensus.
Percent total no. of genes analyzed that showed difference in EST abundance (at probability of difference being by chance less than 0.05 and 0.01) in pair-wise comparisons between “seeds,” “leaves,” “flowers,” and “roots” library pools. The actual no. of genes is in parentheses. Probability calculations were according to Audic and Claverie (1997).