Figure 3.

Detection of Renox mRNA in proximal convoluted tubule cells by in situ hybridization. Antisense (A, C, and E) and sense (B) probes demonstrated specific expression of Renox transcripts within the proximal convoluted tubule cells of the renal cortex (CO). (A–C) Dark-field images in which the positive silver grain signal appears white. (D) Hematoxylin/eosin staining of the field shown in C. (E) Superimposed polarized epi-illumination and bright-field images (in which the signal appears green). High magnification in E shows a strong positive signal in proximal tubule (PT) epithelial cells, whereas glomeruli (GL; marked by arrowheads in C) and distal tubule (DT) epithelial cells are negative for Renox mRNA expression. (A and B) ×14.5 magnification; (C and D) ×60; (E) ×500. OS, outer stripe of the medulla; IS, inner stripe of the medulla; IM, inner medulla. The expression patterns shown were confirmed in two other independent hybridization experiments.