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. 2002 Oct;130(2):918–929. doi: 10.1104/pp.008235

Figure 1.

Figure 1

Diagrams of chimeric gene constructions for transient expression. A, Construct 35SIfSCN contains the 1,028-bp Sh1 first intron flanked by short portions of Sh1 exons 1 and 2 (E1 and E2). The 35S promoter is from CaMV, CAT encodes chloramphenicol acetyltransferase, and the termination region is the 3′ part of the nopaline synthase gene. The transcription start site is designated by the arrow, and the CAT translation initiation codon is indicated as ATG. B, The control construct 35SECN lacks the Sh1 first intron but is otherwise identical to construct 35SIfSCN. C, Deletions of internal portions of the Sh1 first intron were made while retaining all other elements of construct 35SIfSCN. Diagrams of the deletion derivatives indicate the basepairs retained from the 5′ and 3′ ends of the intron.