Table IV.
Sequences T1 and T2 must be within the intron to enhance CAT activity
| Construct | Placement of Test Sequence | CAT Activity
|
|
|---|---|---|---|
| Mean | Range | ||
| % | |||
| 35SCN | None | 2.6 | 1.6–3.5 |
| 35ST1aCN | T1a in 5'-UTR of 35SCN | 3.1 | 1.8–4.4 |
| 35ST2CN | T2 in 5'-UTR of 35SCN | 2.9 | 1.6–4.2 |
| 35SECN | None | 4.1 | 3.5–4.7 |
| 35ST1aECN | T1a in 5'-UTR of 35SECN | 4.0 | 2.0–6.0 |
| 35ST2ECN | T2 in 5'-UTR of 35SECN | 4.2 | 3.6–4.9 |
| DEL8 | None | 57.2 | 48.0–66.3 |
| T1a-DEL8 | T1a in 5'-UTR of DEL8 | 26.7 | 25.0–28.4 |
| T2-DEL8 | T2 in 5'-UTR of DEL8 | 39.0 | 31.3–46.6 |
| DEL7 | Contains T1 within intron | 104.7 | 99.6–109.8 |
| DEL7-T2 | T1 replaced by T2 within intron of DEL7 | 120.8 | 119.0–122.7 |
Enzyme extracts were prepared 24 h post-transfection in two separate experiments. CAT activities are shown relative to construct 35SIfSCN, which contains the entire Sh1 first intron.