Figure 6.

Effects of R59022, an inhibitor of DAGK, on the cold-induced production of PtdOH during an exposure at 0°C. R59022 was added 15 min prior to the cold treatment. Phospholipids were labeled in presence of 53 MBq L⁻¹ [³³P]-PO₄³⁻ for 2 h at 22°C. Lipids were extracted 10 min after the beginning of the cold treatment, and were separated by TLC using the acidic solvent system. PtdOH content was quantified as a fraction of total radiolabeled phospholipids. Values are expressed as the percentage of values obtained in non treated cells (mean of three independent determinations ± sd).