Figure 2.

Suppression of Ca²⁺ sensitivity of the pmc1 vcx1 cnb1 yeast mutant (K667) by full-length and truncated CAX1 and VCAX1 constructs. The CAX constructs are depicted as bars: A black bar represents portions of the CAX1 open reading frame and a gray bar represents portions of the VCAX1 open reading frame (not to scale). Saturated liquid cultures of K667 expressing various plasmids were diluted to the cell densities as indicated, then spotted onto selection medium lacking His (−His) and yeast-extract peptone dextrose (YPD) medium containing 200 mm CaCl₂ (+Ca). Yeast growth at 30°C is shown after 3 d. A representative experiment is shown.