Figure 6.

A, Suppression of Ca²⁺ sensitivity of the pmc1 vcx1 cnb1 yeast mutant (K667) by sCAX1 with N-terminal fusions of either the wild-type NRR of VCAX1 (VN:sCAX1) or the NRR of VCAX1 containing an MRH to LRL double amino acid change (VN-2:sCAX1). The CAX constructs are depicted as bars: A black bar represents portions of the CAX1 open reading frame and a gray bar represents portions of the VCAX1 open reading frame (not to scale). The assay conditions were the same as in Figure 2, except yeast growth is shown after 2 d. B, Suppression of Ca²⁺ sensitivity of the pmc1 vcx1 cnb1 yeast mutant (K667) by full-length and truncated CAX1 compared with a mutant CAX1 with a single Leu-30 to His amino acid change (CAX1-L30H). The CAX constructs are depicted as bars: A black bar represents portions of the CAX1 open reading frame and a white bar represents portions of the CAX3 open reading frame (not to scale). The assay conditions were the same as in Figure 2. Representative experiments are shown in A and B.