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. 2002 Nov;130(3):1221–1229. doi: 10.1104/pp.102.010918

Figure 2.

Figure 2

Phosphorylation of BES1/BZR1 by BIN2 in vitro. A, BIN2 can phosphorylate both BES1 and BZR1 in vitro. GST-BES1 (top) or GST-BZR1 (bottom) fusion proteins were mixed with the wild-type BIN2 (lane 1), the Lys-69-Arg-mutated GST-BIN2 (lane 2), or the bin2-1-mutated GST-BIN2 (lane 3) to measure protein phosphorylation activity as described in “Materials and Methods.” B, GST-BRI1CK was unable to phosphorylate BES1 or BZR1 protein. C, The phosphorylation of BES1 or BZR1 by BIN2 can be inhibited by lithium. For A through C, the amount of proteins used in the kinase assays is indicated by Coomassie Blue staining in the top strip above the white dividing line, whereas the level of proteins phosphorylation is shown by autoradiography in the bottom strip.