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. 2002 Nov;130(3):1221–1229. doi: 10.1104/pp.102.010918

Figure 3.

Figure 3

BIN2 phosphorylation of BES1 and BZR1 via a novel mechanism. A, Phosphorylation of BES1 and BZR1 by BIN2 does not require priming phosphorylation. CIP-treated GST-BES1 or GST-BZR1 was incubated with GST-BIN2 or with a mutated GST-BIN2 fusion protein containing Arg-80-Ala mutation, and assayed for protein phosphorylation as described in the “Materials and Methods.” B, Both GST-BES1 and GST-BZR1 proteins can be phosphorylated by an MBP-BIN2 fusion kinase. C, FRATide has no inhibitory effect on the BIN2 phosphorylation of either BES1 or BZR1. For all panels, the amount of protein used for the kinase assays are indicated in the top strip, whereas the levels of protein phosphorylation are shown in the bottom strip.