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. 2002 Nov;130(3):1230–1240. doi: 10.1104/pp.011353

Figure 3.

Figure 3

Functional complementation of fission yeast temperature sensitive UbcP4-140 strain. A, Schematic representation of the constructs used for yeast complementation. All constructs were put into the pREP1 vector. Open and hatched boxes represent the N-terminal extension domains of UBC19 and Sp_UbcP4, respectively. The gray boxes represent UBC19 core domain. The catalytic Cys or the Ser (in the dominant negative version of the protein) residues are also represented. B, The yeast mutant strain was transformed with either an empty pREP1 vector or with the pRep1 vector expressing wild-type UBC19 (1) or chimeric UbcP4(Nt)/UBC19 (2) or the dominant negative UBC19 (C/S; 3). The wild-type yeast strain (WT) and the transformed UbcP4-140 strains were grown either at permissive (28°C) or restrictive (37°C) temperature.