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TUNEL staining of BAT. (a) Lean control mice acclimated to 28°C, the thermoneutral temperature. (Inset) Intestinal villi showing apoptotic apical epithelial cells as positive controls. (b) Lean control mice acclimated to cold temperature (10°C for 10 days). (c) ob/ob mice (wild type at TNFR loci). (Inset) A negative adipose cell nucleus (arrow) between two apoptotic nuclei. (d) ob/ob p55−/−. (e) ob/ob p75−/−. (f) ob/ob p55−/−p75−/− mice. [a–f and Inset a, ×125; Inset c, ×790.]
