RNA gel-blot analysis of β-1,6-glucanase transcripts. A, Lane 1, E− P. ampla; lane 2, E+ P. ampla; lane 3, Neotyphodium sp. grown on 0.5% (w/v) pustulan; lane 4, Neotyphodium sp. grown on 0.5% (w/v) laminarin. Fifteen micrograms of total RNA was used from E− and E+ P. ampla leaf sheaths. Ten micrograms of total RNA was used from the Neotyphodium sp. fungal cultures. B, Lane 1, E− P. ampla; lane 2, E+ P. ampla; lane 3, E+ Chewings fescue; lane 4, E+ tall fescue; lane 5, Chewings fescue 1117 artificially infected with the Neotyphodium sp. Four micrograms of poly(A+) RNA was used for each sample.