The effect of varying extracellular (cis) Ca2+ concentration on the unitary current versus voltage relationships for the VIC channel in the plasma membrane of wheat roots. Assay solutions contained 100 mm NaCl plus 0 (●), 0.1 (○), 0.5 (▪), 1 (□), 2 (▴), 5 (▵), or 10 (▾) mm CaCl2 (cis) and 100 mm NaCl (trans). Curves were derived from a 3B2S model for cation permeation using the parameters given in Table I.