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. 2002 Nov;130(3):1487–1496. doi: 10.1104/pp.007971

Figure 4.

Figure 4

EMSA for binding of bean AZ transcription factors to a 24-bp Z-BAC+ element. A, Sequence for wild-type (WT) and mutant (Mu) oligonucleotides. B, Transcription factors tested were in vitro transcribed and translated from three full-length TGA-type bZIP cDNAs isolated from a bean AZ cDNA library and competition experiments included 100-fold excess of unlabeled wild-type (WT) Z-BAC+ element or mutant Z-BAC+ element (Mu) or no unlabeled DNA added (−). First lane, Incubation where no protein or competitive DNA was added.