Figure 4.

⁸⁶Rb influx measurements in cal-res and non-cal-res portions of the lightest, R1, fraction of SS RBCs, in the presence and absence of ouabain and high (1 mM) bumetanide. Before measurements, the isolated, dehydrated non-cal-res cells were replenished with KCl and rehydrated. A and B show two patterns of influx seen in four experiments. ○, cal-res RBCs; ●, cal-res RBCs + ouabain; □, non-cal-res RBCs; ■, non-cal-res RBCs + ouabain. The experiment in C is typical of several showing the effects of added bumetanide on ouabain-treated cal-res and non-cal-res RBCs. □, cal-res RBCs + ouabain; ■, cal-res RBCs + ouabain + bumetanide; ▿, non-cal-res RBCs + ouabain; ▾, non-cal-res RBCs + ouabain + bumetanide.