Table 1.
Na and K content of SS val-res RBC
| Experiment/donor | Content, mmol/liter of cell water*
|
|
|---|---|---|
| Na+ | K+ | |
| J.M. | 122.8 | 56 |
| L.M. | 104.1 | 29.5 |
| K.C. | 107.2 | 62.4 |
| J.M. | 99.4 | 34.5 |
| R.D. | 90.5 | 70.9 |
| J.M. | 78.6 | 30.5 |
| J.M. | 89.8 | 31.8 |
| J.N. | 104.2 | 24.9 |
| J.M.† | 79.6 | 22.7 |
| Mean ± SD | 97.4 ± 13.4 | 40.4 ± 16.8 |
The light-density fractions (R1, or R1 and R2 combined) of SS RBC were K+-permeabilized by exposure to valinomycin, and the val-res fractions were isolated.
*
Cell water: for 20 g of Hb/dl of RBC, with specific volume of Hb = 0.75 ml/g, Hb = 15 ml/dl and water = 85 ml/dl of RBC. Thus [mmol (Na or K)/20 g of Hb]/0.85 = (Na or K)/liter of cell water.
†
Choline+ replaced Na+ in buffers, from the time of the first RBC washes, after sampling.