Figure 3.

RNA gel-blot (20 μg of total RNA lane⁻¹) analyses showing expression patterns of transporters (hexose transporter and PM H⁺-ATPase) and putative regulatory (ZmMADS1 and Grf1) genes in developing pollen at early (E) and late (L) stages from maintainer, male-sterile, and F₁-restored genotypes. The same blot or parallel blots run under identical conditions were hybridized as described in “Materials and Methods.”