Figure 1.
pBAR-GUS (A), pAdh1-ASOMT (B), and pBAR (C) constructs used for maize transformation. pAdh1, Promoter of maize Adh1 gene; iAdh1, first intron of Adh1 maize gene; CaMV35S, cauliflower mosaic virus (CaMV) 35S RNA promoter; PAT or BAR, coding sequence of phosphinotricin acetyltransferase gene conferring resistance to phosphinotricin (bialaphos [Basta]); Tnos, terminator of the nopaline synthase gene; AS OMT, partial sequence (850 bp) of the maize COMT in AS orientation; GUS, coding sequence of uidA gene.