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. 2002 Dec;130(4):1675–1685. doi: 10.1104/pp.012237

Figure 2.

Figure 2

GUS expression in different organs of 30-d-old transgenic maize transformed with the Adh1 promoter construct (pBAR-GUS; Fig. 1A). A and B, Cross section of the nodal region of maize stem. GUS staining is restricted to vascular bundles (A) and more precisely in cells surrounding the protoxylem (B, open arrowhead). C, Root cross sections. GUS activity is mainly located in protoxylem cells. D through G, Cross sections of rolled leaves around the internodes. In proximal leaves (youngest), GUS activity is located in differentiating sclerenchyma cells and in cells surrounding the protoxylem (D). In distal (older) leaves, small and large vascular strands exhibit GUS activity (E). Higher magnifications indicate GUS activity in protoxylem cells of small vascular strands (F) and in phloem and companion cells of large vascular strands (G). px, Protoxylem; s, sclerenchyma; pp, protophloem; ps, procambial strands. Magnification bar = 50 μm (A–C) and 100 μm (D–G).