Figure 3.
Effect of Al stress on cell elongation and division-related genes. A, RNA-blot hybridization showing different expression profiles during Al treatment of rye cv Blanco roots over a 48-h period. Total RNA was isolated from the same Al stress experiment. The first (0 h) and last (48 h) lanes correspond to unstressed plants (0 mg L−1). The rRNAs of one representative blot stained with methylene blue show RNA integrity and uniform loading (Herrin and Schmidt, 1988). TIP, aquaporin; SMT3, ubiquitin-like protein SMT3. B, Quantitation of mRNA levels. The hybridization signals obtained from the genes were weighted against those obtained from the scan image of the 25S rRNA stained with methylene blue to correct for minor differences in RNA loading, normalized to that at 0 h (which was set at 1 for each gene) and plotted against time to compare changes in gene expression.