Figure 4.

Effect of high CO₂ on AtAMT2 expression. Plants were grown in soil in a normal atmosphere (400 μL L⁻¹ CO₂) for 3 weeks before a subset of plants were transferred to high CO₂ (800 μL L⁻¹) conditions. Twenty micrograms of total RNA isolated from whole plants was separated, blotted, and probed with an antisense riboprobe specific to the 3′ part of AMT2. Lower, Ethidium bromide-stained gel before blotting.