Figure 1.

Fluorescent images of epidermal cells of seedlings of transgenic Arabidopsis plants expressing GFP-HDEL. Arabidopsis plants were transformed with a p35S::sp-gfp-hdel gene encoding the signal peptide of pumpkin 2S albumin and GFP followed by a 12-amino acid sequence including an ER retention signal, HDEL (Mitsuhashi et al., 2000). Six-day-old (B and C) and 12-d-old (A, D, and E) seedlings were inspected with a fluorescence microscope. ER bodies (0.5-μm diameter × approximately 5 μm long) and the fluorescent ER network were found in the epidermal cells of cotyledons (A and B), hypocotyls (C and D), and roots (E) of the seedlings. Bars = 20 μm.