Figure 7.
An immunoblot showing BiP content of rosette leaves was not affected by chemical treatments. Extracts were prepared from the wild-type (Wt) and transgenic (GFP-HDEL) leaves treated with water, 50 μm MeJA, and 50 μm MeJA plus 20 μL L−1 ethylene (ET) for 36 h. The extracts were subjected to SDS-PAGE and immunoblotted with anti-BiP antibodies. Lanes 1 and 2, Wild type after water treatment; lanes 3 and 4, transgenic plants after water treatment; lanes 5 and 6, wild type treated with MeJA; lanes 7 and 8, transgenic plants treated with MeJA; lanes 9 and 10, wild type treated with MeJA plus ET; lanes 11 and 12, transgenic plants treated with MeJA plus ET.