TABLE 1.
Strains used in this study
| Strain name | Genotype | Source |
|---|---|---|
| DSR741-3B | MATaleu2-2,112 his3-11,15 trp1 ura3 | Ruby and Szostak (1985) |
| Strains derived from DSR741-3B | ||
| TSR30-15 | RNR3-lacZ (1 copy) | Ruby and Szostak (1985) |
| TSR30-23 | RNR3-lacZ (2–4 copies) | Ruby and Szostak (1985) |
| T19 | RNR3-lacZ (2–4 copies) + pSR50 | Ruby and Szostak (1985) |
| TSG1 | RNR3-lacZ (1 copy), wtm1wtm2Δ∷HIS3 | This study |
| TSG10 | RNR3-lacZ (1 copy) | This study |
| TSG12 | RNR3-lacZ (1 copy), wtm1Δ∷HIS3 | This study |
| TSG19 | RNR3-lacZ (1 copy) + pSG5 (WTM1) + pRS423 | This study |
| TSG20 | RNR3-lacZ (1 copy) + pSG5 (WTM1) + pSR61 (WTM2) | This study |
| TSG21 | RNR3-lacZ (1 copy) + pRS425 + pRS423 | This study |
| TSG23 | RNR3-lacZ (1 copy) + pRS425 + pSR61 (WTM2) | This study |
| TSG41 | pRS424 | This study |
| TSG42 | pSG12 (WTM2 + WTM1) | This study |
| TSG78 | pSG20 (RNR3-CYC1-lacZ) + pRS424 | This study |
| TSG80 | pSG20 (RNR3-CYC1-lacZ) + pSG12 (WTM2 + WTM1) | This study |
| TSG85 | RNR3-lacZ (1 copy) + pRS425 + pG-3 | This study |
| TSG86 | RNR3-lacZ (1 copy) + pRS425 + pSGX (GPD-WTM2) | This study |
| TSG88 | RNR3-lacZ (1 copy) + pSG5 (WTM1) + pG-3 | This study |
| TSG89 | RNR3-lacZ (1 copy) + pSG5 (WTM1) + pSGX (GPD-WTM2) | This study |
| TSG94 | RNR3-lacZ (1 copy) + pG-3 | This study |
| TSG95 | RNR3-lacZ (1 copy) + pSGX (GPD-WTM2) | This study |
| TSG97 | RNR3-lacZ (1 copy), wtm1wtm2Δ∷HIS3 + pG-3 | This study |
| TSG98 | RNR3-lacZ (1 copy), wtm1wtm2Δ∷HIS3 + pSGX (GPD-WTM2) | This study |
| TSG100 | RNR3-lacZ (1 copy), pSGX (GPD-WTM2) | This study |
| TSG107 | pSGX (GPD-WTM2) | This study |
| TSG125 | DIN7-lacZ (1 copy) + pRS423 | This study |
| TSG126 | DIN7-lacZ (1 copy) + pSR54 (WTM2 + WTM1) | This study |
| TSG133 | pSGX (GPD-WTM2) | This study |
| TSG134 | pG-3 | This study |
| TSG1257 | RNR3-lacZ (1 copy), wtm2Δ∷LEU2 | This study |
| TSR2048 | RNR3-lacZ (1 copy) + pSR54 (WTM2 + WTM1) | This study |
| TSR2051 | RNR3-lacZ (1 copy) + pRS423 | This study |
| TSR2147 | pSE788 (RNR2-CYC1-lacZ) + pRS424 | This study |
| TSR2148 | pSE836 (RNR1-CYC1-lacZ) + pRS424 | This study |
| TSR2150 | pSE788 (RNR2-CYC1-lacZ) + pSG12 (WTM2 + WTM1) | This study |
| TSR2151 | pSE836 (RNR1-CYC1-lacZ) + pSG12 (WTM2 + WTM1) | This study |
| TSR2167 | pSE788 (RNR2-CYC1-lacZ) | This study |
| TSR2169 | pSE836 (RNR1-CYC1-lacZ) | This study |
| TSR2170 | wtm1wtm2D∷HIS3 + pSE788 (RNR2-CYC1-lacZ) | This study |
| TSR2172 | wtm1wtm2D∷HIS3 + pSE836 (RNR1-CYC1-lacZ) | This study |
| TSR2386 | wtm2Δ∷LEU2 + pG-3 | This study |
| TSR2387 | wtm2Δ∷LEU2 + pJW1 (GPD-WTM2-TAP) | This study |
| TSR2425 | pLGSD5 (GAL10-CYC1-lacZ) + pRS424 | This study |
| TSR2427 | pLGSD5 (GAL10-CYC1-lacZ) + pSG12 (WTM2 + WTM1) | This study |
| TSR2441 | RNR3-lacZ + pKL6 (GPD-WTM1) | This study |
| TSR2451 | wtm2Δ∷LEU2 + pSR336 (GPD-wtm2-TAP) | This study |
| Y80 | MATa, can1-100, ade2-1, his3-11, leu2-3,112, trp1-1, ura3-1 | Huang et al. (1998) |
| Strains derived from Y80 | ||
| Y301 | rad53-21 | Huang et al. (1998) |
| TSG46 | RNR3-lacZ (1 copy) + pRS424 | This study |
| TSG47 | RNR3-lacZ (1 copy) + pSG13 (WTM2 + WTM1) | This study |
| TSG48 | rad53-21, RNR3-lacZ (1 copy) + pRS424 | This study |
| TSG49 | rad53-21, RNR3-lacZ (1 copy) + pSG13 (WTM2 + WTM1) | This study |