Figure 2.—

The sah1L459F mutation suppresses PAI2 silencing. (A and B) Two-week-old seedlings of the indicated genotypes grown on agar medium were photographed under visible (top) or UV (bottom) light, with sah1 indicating sah1L459F. (A) Representative seedlings of mutants isolated from the PAI2 silencing suppressor screen. (B) Representative T2 generation seedlings of Ws pai1 sah1L459F transformed with either an SAH1 (T-SAH1) or a control SHM (T-SHM) genomic transgene. (C) Genomic DNAs prepared from 4-week-old plants of the indicated genotypes were cleaved with HpaII (H) or MspI (M) and used for Southern blot analysis with a PAI probe. P1–P4 is PAI1–PAI4, P2 is PAI2, and P3 is PAI3, with asterisks indicating the positions of species methylated at PAI-internal sites. WT is Ws pai1, sah1 is Ws pai1 sah1L459F, and the remaining strains are Ws pai1 sah1L459F transformed with the indicated transgenes, corresponding to the transgenic lines shown in B.