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The sah1L459F mutation reduces PAI non-CG methylation. Genomic DNA prepared from 4-week-old plants of the indicated genotypes was cleaved with (A) HincII, (B) HpaII (left) or MspI (right), or (C) ClaI and used for Southern blot analysis with a PAI probe. P1–P4 is PAI1–PAI4, P2 is PAI2, and P3 is PAI3, with asterisks indicating the positions of species methylated at PAI-internal sites. All strain backgrounds carried the pai1 mutation. WT is Ws pai1 and sah1 is Ws pai1 sah1L459F.
