Figure 4.—

Genomic bisulfite methylation sequencing data for PAI1 and PAI2 proximal promoter regions in the sah1L459F mutant. Eight independent top strand clones were sequenced for PAI1 or PAI2 from bisulfite-treated DNA of Ws pai1 sah1L459F (sah1) as previously described (Melquist and Bender 2003). The percentage of 5-methyl-cytosines detected out of total cytosines available within the region of PAI sequence identity (344 bp for PAI1 or 338 bp for PAI2) is shown, divided into the contexts CG (solid bars), CNG (open bars), and other contexts (shaded bars). For comparison, previously determined methylation patterns for wild-type Ws (WT) (Luff et al. 1999), Ws pai1 suvh4R302* (suvh4) (Malagnac et al. 2002), and Ws pai1 cmt3G456D (cmt3) (Bartee et al. 2001) are shown.