Figure 5.—

The sah1L459F mutation reduces non-CG methylation on transposon sequences, similarly to suvh4 and cmt3 mutations. Genomic DNA prepared from 4-week-old plants of the indicated genotypes was cleaved with the indicated restriction enzymes and used for Southern blot analysis with the indicated probes. (A) DNA digested with MspI and HindIII and probed for Mu1. (B) DNA digested with MspI and probed for Ta3. (C) DNA digested with MspI and probed for MEA-ISR. (D) DNA digested with HpaII (left) or MspI (right) and probed for the 180-bp centromere repeat. WT is the wild-type Ws, sah1 is sah1L459F, and drm1+2 is Ws drm1 drm2. Arrowheads in left margins indicate the positions of fully cleaved species.