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The effect of chelators on the AtHO1 reaction
| Chelator (2 mm) | BV | Relative Activity |
|---|---|---|
| nmol min−1 | % | |
| Desferrioxamine | 1.71 | 100 |
| Tiron | 0.81 | 47.3 |
| o-Phenanthrolin | 1.71 | 100 |
| EDTA | 0.69 | 40.4 |
| No chelator | 0.43 | 25.1 |
| No enzyme | 0.02 | 1.1 |
Reactions were carried out for 10 min using the standard heme oxygenase assay as described in “Materials and Methods” with desferrioxamine replaced by different chelators.
