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The effect of reductants on the AtHO1 reaction
| Reductants (5 mm) | BV | Relative Activity |
|---|---|---|
| nmol min−1 | % | |
| Ascorbate | 1.46 | 100 |
| Iso-ascorbate | 1.18 | 81.0 |
| Dehydroascorbate | 0.23 | 15.8 |
| Dithothreitol | NDa | ND |
| Trolox | 0.81 | 55.3 |
| None | 0.14 | 9.8 |
Reactions were carried out for 10 min using the standard heme oxygenase assay as described in “Materials and Methods” with ascorbate replaced by different reductants.
ND, Not detectable.
